Five actives, and why each one is here.

Two formulas carry five actives between them.

47 published human studies behind the five actives See them →
See the ingredient records See how we verify
The two Serene Biotics cartons side by side on a stone ledge: Daily Calm Focus in cream and Nightly Rest & Restore in deep teal
  • Split morning and night
  • DR7, a named strain
  • Bimuno® GOS at the studied dose
  • Magnesium bisglycinate, not oxide
  • Split morning and night
  • DR7, a named strain
  • Bimuno® GOS at the studied dose
  • Magnesium bisglycinate, not oxide

Underdosing is the category's oldest trick.

Each active here sits at an amount published human research actually used. Nothing is dropped in at a token amount to make the label look longer.

An ingredient that cannot carry its own research does not go in.

Daily Calm Focus dissolved in a glass of cool water, an opaque bright citrus yellow

We did not invent any of this.

across the five actives

47

published human studies

Bimuno® GOS 19, L‑theanine 12, magnesium 6, the B vitamins 6, and L. plantarum DR7 4. Open any of them on the science page and you get the design, the dose, and what it measured.

See the studies
Golden powder blooming through water against deep teal
Golden powder blooming through water against deep teal

Your gut and your brain are always talking.

That conversation runs both ways. A rough stretch of sleep shows up in your digestion, and an unsettled gut shows up in how clearly you think. The protocol works on both ends of it, once in the morning and once at night.

The gut-brain axis: a teal human silhouette with a gold vagus-nerve pathway connecting the brain to the gut and its microbiome
The gut-brain axis: a direct line between the brain and the gut microbiome.

Gut to brain, one stage at a time

  1. You take it

    One precision-dosed stick pack in cool water, morning and night.

  2. It reaches your gut

    L. plantarum DR7 and the Bimuno® prebiotic arrive in the gut, where the gut-brain conversation begins.

  3. Your microbiome responds

    The prebiotic feeds your beneficial bacteria, which produce the short-chain fatty acids and messengers the gut uses to signal.

  4. The signal travels up

    The vagus nerve carries that signal from gut to brain. This is the gut-brain axis in action.

  5. You feel it

    Calm focus by day, restful sleep by night. Restoration is what you feel.

What researchers measured for DR7 against placebo

Improvement at week 8 · higher is better

DR7 group +8.5
Placebo +5.0

At week 8, adults taking the DR7 strain improved more than placebo on stress-related scores, a statistically significant difference.

See the full study

What researchers measured for the DR7 probiotic strain in a clinical trial, reported as published. Individual results vary.

See all the studies and the full data
The prebiotic half: Bimuno® GOS at 1.39 g a serving
When digestive comfort begins Bimuno® GOS, 1.37 g · Vulevic 2018, n=83Baseline StartLess bloating, gas & abdominal discomfort p < 0.001 vs placebo Week 1Comfort sustained Week 2 A 7-week randomized, placebo-controlled crossover trial at 1.37 g a day.

In a 7-week randomized, placebo-controlled trial (Vulevic 2018, n=83, at the 1.37 g dose), the clinically studied Bimuno® GOS prebiotic supported digestive comfort, with less bloating, gas and abdominal discomfort vs placebo by the first week (p < 0.001), sustained through week 2. Bimuno® is a trademark of Clasado Limited.

See the Bimuno studies

What researchers measured for the Bimuno® GOS prebiotic in clinical trials, reported as published.

See the Bimuno studies

Most labels stop at a name and an amount.

Morning

L‑theanine

Green tea's calm-focus amino acid, without the caffeine.

200mgper serving

Source

Form. L‑theanine, 200 mg per serving.

Supplier. Confirmed with first lot · at launch

Dose Morning

200 mg in the morning serving, the same amount published L-theanine research used.

Clinically studied to support calm focus and clear thinking, without the jitters.

Study

Hidese 2019 (Nutrients), one of 12 published L-theanine human studies, studied the 200 mg dose used here for calm, relaxed-alertness context.

Read the science

Batch

Every finished lot must clear third-party testing for L‑theanine identity, potency, and purity before release.

See testing & batch records
Morning

Active B‑complex

Five B vitamins in forms your body can use straight away.

250mcgB12 per serving

Source

Form. B1 25 mg, B2 15 mg, B5 50 mg, B6 as P‑5‑P 10 mg, B12 as methylcobalamin 250 mcg.

Supplier. Confirmed with first lot · at launch

Dose Morning

B6 and B12 arrive here already in their coenzyme forms, P‑5‑P and methylcobalamin, so your body does not have to convert them first.

These are the B vitamins your body runs everyday energy metabolism and nervous-system support on.

Study

Kennedy 2010 (Nutr J), n=215, a ~1-month trial of a B-complex with vitamin C and minerals, studied for energy metabolism and a healthy stress response.

Read the science

Batch

Every finished lot must clear third-party testing for the identity and potency of each of the five B vitamins before release.

See testing & batch records
Night

Magnesium bisglycinate

Magnesium bound to glycine, and not a milligram of melatonin.

200mgelemental · serving

Source

Form. 200 mg elemental magnesium per serving, provided as magnesium bisglycinate.

Supplier. Confirmed with first lot · at launch

Dose Night

Most of the category uses oxide because it is the cheapest form to buy. Nightly Rest & Restore uses bisglycinate at 200 mg of elemental magnesium in every evening serving.

Study

Schuster 2025 (Nat Sci Sleep), n=155, one of 6 published magnesium human studies, studied magnesium bisglycinate, the same fully chelated form in the evening formula, for relaxation and sleep quality.

Read the science

Batch

Every finished lot must clear third-party testing for elemental magnesium content and purity before release.

See testing & batch records
Day & night

L. plantarum DR7

One named strain, with its own human trials.

1BCFUper serving

Source

Maker. Kaneka, the supplier that owns and characterizes the DR7 strain.

Form. Live Lactiplantibacillus plantarum DR7, a named and characterized strain. 1 billion CFU per serving, 2 billion across the morning and evening protocol.

Dose Day & night

1 billion CFU per serving, 2 billion across the morning and evening protocol. Named to its exact strain code, not a generic L. plantarum, so every claim traces to a study of this specific strain.

Study

Chong 2019 (Benef Microbes), n=111, a randomized controlled human trial, studied this strain at 1 billion CFU for a healthy stress response.

Read the science

Batch

Every finished lot must clear third-party testing for DR7 strain identity and live count before release.

See testing & batch records
Day & night

Bimuno® prebiotic

The fiber that feeds the strain you just took.

1.39gper serving

Source

Maker. Bimuno®, the branded GOS prebiotic from Clasado.

Form. Bimuno® galactooligosaccharides (GOS), 1.39 g in every serving, morning and evening.

Dose Day & night

1.39 g in every serving, morning and evening. It is paired with the DR7 strain for a reason. A probiotic only works if something feeds it, and this is what feeds it.

One of the most-studied prebiotic fibers, clinically studied to support the growth of beneficial Bifidobacterium and digestive comfort.

Study

Vulevic 2018 (Neurogastroenterol Motil), n=83, studied Bimuno® GOS at 1.37 g a day.

Read the science

Batch

Every finished lot must clear third-party testing for GOS identity, potency, and purity before release.

See testing & batch records

Each active is then blended and filled to its label amount into the precision-dosed morning or evening serving.

Supplier and origin details appear here as each is confirmed and tied to its batch record. We leave unverified sourcing fields blank rather than guessing.

Contains milk: Bimuno® prebiotic is made from lactose. Bimuno® is a trademark of Clasado Limited.

The other two questions worth asking.

  1. 1

    What is in it You are here

    Five actives, each with its amount, its maker, and the trial behind it.

  2. 2

    Why it is safe

    What every finished lot must clear before it can be released.

    See how we verify
  3. 3

    The science

    The published human studies behind every active, with the amount used in this formula.

    Read the science

This is the part where you decide.

Two precision-dosed formulas, one for your morning and one for your night.

  • 30-day money-back guaranteeFull refund within 30 days of delivery.
  • $130 founding welcome kitIncluded with the 3- and 6-month protocol.
  • Clinician-advisedDr. Yeragunta, MD, medical advisor.
Shop the protocol Explore the science