One protocol,
morning and night.

Two cartons, one shared gut-brain base, a different active on top.

Digestive comfort in week 1 · Vulevic 2018 · 1.39 g of GOS in every serving

The two Serene Biotics formulas side by side: Daily Calm Focus and Nightly Rest & Restore

Your gut talks to your brain, all day long.

That conversation runs mostly from the gut upward, which is why the protocol starts there. It feeds the bacteria already living in your gut with prebiotic fiber.

Feed those bacteria at breakfast and they are still working at midnight.

Explore the science
A drawn diagram in gold line work tracing the vagus nerve path from the gut to the brain
2.75g of prebiotic fiber a day at least, the food those bacteria live on.

What each ingredient is actually doing.

Calm that does not slow you down. Daily Calm Focus · L-theanine 200 mg + active B-complex See the proofClose

L-theanine is the amino acid in green tea, and it is why a cup of tea feels steady where coffee feels sharp. Daily Calm Focus carries 200 mg of it, the amount used in the published research. The active B-complex sits alongside it, with B6, B12, and folate already in their coenzyme forms, so your body has no conversion step to do first.

Clinically studied for calm, relaxed alertness without sedation, and for a healthy stress response. Hidese 2019, 30 adults, 4 weeks, at the same 200 mg daily amount.

Explore the science
The magnesium worth taking at night. Nightly Rest & Restore · Magnesium bisglycinate, 200 mg elemental See the proofClose

Magnesium is not one ingredient. Most products use oxide because it is the cheapest, and it is the reason magnesium has the reputation it does. Nightly Rest & Restore uses bisglycinate, magnesium bound to glycine, at 200 mg of elemental magnesium in every evening serving. Gentle enough to take right before bed, which is the entire point of an evening formula. No melatonin, so nothing to sleep off in the morning.

Schuster 2025. A randomized, double-blind, placebo-controlled trial of magnesium bisglycinate in 155 adults over 4 weeks.

Explore the science
A probiotic, and the fiber that feeds it. Both formulas · L. plantarum DR7 at 1 billion CFU with Bimuno® GOS See the proofClose

A probiotic can only do so much on its own. DR7 is a named, characterized strain of L. plantarum, 1 billion CFU per serving, and it arrives with 1.39 g of active Bimuno GOS, the prebiotic fiber it lives on. Pairing the two is what makes this a synbiotic, and both formulas carry it.

Vulevic 2018. 83 adults at that amount reported better digestive comfort inside the first week.

See every ingredient
Nothing will ship until the lab clears it. Protocol record · Eurofins identity, potency, and purity testing See the proofClose

Eurofins will test every batch for identity, potency, and purity, and no batch will leave the warehouse without clearing it. At launch, the code printed on your carton will open the record for the batch in your hand.

Check the Verify page

Your body runs on a clock. So does the protocol.

You do not need the same thing at 8 in the morning that you need at 10 at night. The synbiotic base stays constant in both cartons. The timed active is what moves.

A Daily Calm Focus stick pack, the morning formula
Morning

Daily Calm Focus

Steady, calm focus through the morning, with no caffeine anywhere in the formula.

  • L-theanine 200 mg
  • Active B-complex, with B6, B12, and folate
  • L. plantarum DR7, 1 billion CFU
  • Bimuno® GOS, 1.39 g
A Nightly Rest and Restore stick pack, the evening formula
Evening

Nightly Rest & Restore

Made to support relaxation and restful sleep, with no melatonin, so there is nothing to shake off in the morning.

  • Magnesium bisglycinate, 200 mg elemental
  • L. plantarum DR7, 1 billion CFU
  • Bimuno® GOS, 1.39 g

How long before you feel something?

Morning · Daily Calm Focus

  1. Day 1Calm, steady focus the same day you start.
  2. Week 1Digestive comfort begins to settle.
  3. Weeks 2 to 4The gut-brain base builds with daily use.
  4. Weeks 8 to 12This is where consistency pays.

Evening · Nightly Rest & Restore

  1. Night 1Your first wind-down serving.
  2. Week 1Digestive comfort begins to settle.
  3. Weeks 2 to 4Restful-sleep support builds with the routine.
  4. Weeks 8 to 12The routine is doing the work by now.

L-theanine and magnesium do their work the day you take them. The synbiotic base is slower by nature, because feeding a gut population takes weeks rather than hours. Individual results vary.

Two pours a day, about ten seconds each.

  1. 01

    Morning, in cool water

    Stir one Daily Calm Focus serving into cool water with breakfast. Peach Mango, and nothing to swallow.

  2. 02

    Evening, as you wind down

    Stir Nightly Rest & Restore into cool water while the day is closing out. Hibiscus Vanilla.

  3. 03

    Then again tomorrow

    Take both every day. Consistency is the mechanism.

The morning formula dissolved in a glass of water, an opaque bright citrus yellow
Morning · the real pour Peach Mango

The rest of it you have to feel for yourself.

Pre-order both formulas together. You are charged today and your first delivery ships Fall 2026.

60 Servings in one Protocol order30 mornings and 30 nights. A full month of both formulas in one order.
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